T7

Part:BBa_K606049

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-09-18)

Lambda phage attL recombination site

This sequence is the AttL recombination sequence from the lambda phage. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3.

The idea is that this part can be used in combination with the part K606056. When expressing the Xis protein, the stop can be excised, and the transcription recovered.

Excision xis-reporter.jpg


The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None